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首页> 外文OA文献 >Characterization of an F1 Deletion Mutant of Yersinia pestis CO92, Pathogenic Role of F1 Antigen in Bubonic and Pneumonic Plague, and Evaluation of Sensitivity and Specificity of F1 Antigen Capture-Based Dipsticks▿
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Characterization of an F1 Deletion Mutant of Yersinia pestis CO92, Pathogenic Role of F1 Antigen in Bubonic and Pneumonic Plague, and Evaluation of Sensitivity and Specificity of F1 Antigen Capture-Based Dipsticks▿

机译：鼠疫耶尔森菌CO92的F1缺失突变体的表征，F1抗原在牛瘟和肺炎鼠疫中的致病作用以及基于F1抗原捕获的量油尺的敏感性和特异性评估▿

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We evaluated two commercial F1 antigen capture-based immunochromatographic dipsticks, Yersinia Pestis (F1) Smart II and Plague BioThreat Alert test strips, in detecting plague bacilli by using whole-blood samples from mice experimentally infected with Yersinia pestis CO92. To assess the specificities of these dipsticks, an in-frame F1-deficient mutant of CO92 (Δcaf) was generated by homologous recombination and used as a negative control. Based on genetic, antigenic/immunologic, and electron microscopic analyses, the Δcaf mutant was devoid of a capsule. The growth rate of the Δcaf mutant generally was similar to that of the wild-type (WT) bacterium at both 26 and 37°C, although the mutant's growth dropped slightly during the late phase at 37°C. The Δcaf mutant was as virulent as WT CO92 in the pneumonic plague mouse model; however, it was attenuated in developing bubonic plague. Both dipsticks had similar sensitivities, requiring a minimum of 0.5 μg/ml of purified F1 antigen or 1 × 105 to 5 × 105 CFU/ml of WT CO92 for positive results, while the blood samples were negative for up to 1 × 108 CFU/ml of the Δcaf mutant. Our studies demonstrated the diagnostic potential of two plague dipsticks in detecting capsular-positive strains of Y. pestis in bubonic and pneumonic plague.
机译：我们通过使用实验性感染鼠疫耶尔森氏菌CO92的小鼠的全血样品，评估了两种商业性的基于F1抗原捕获的免疫色谱试纸，耶尔森氏菌（F1）Smart II和Plague BioThreat Alert测试条，以检测鼠疫杆菌。为了评估这些量油尺的特异性，通过同源重组产生了框内F1缺失的CO92突变体（Δcaf），并将其用作阴性对照。根据遗传，抗原/免疫学和电子显微镜分析，Δcaf突变体没有胶囊。 Δcaf突变体的生长速率在26和37°C下通常与野生型（WT）细菌的生长速率相似，尽管该突变体的生长在37°C的后期阶段略有下降。在肺鼠疫鼠模型中，Δcaf突变体的毒性与WT CO92相同。然而，它在发展中的鼠疫中有所减弱。两种试纸都具有相似的敏感性，至少需要0.5μg/ ml的纯化F1抗原或1×105至5×105 CFU / ml的WT CO92才能获得阳性结果，而血液样本中的阴性结果则高达1×108 CFU / ml的Δcaf突变体。我们的研究表明，在鼠疫和肺炎鼠疫中，两种鼠疫试纸在检测鼠疫耶尔森氏菌荚膜阳性菌株中具有诊断潜力。

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